Experiments showing how life can naturally arise out of inorganic materials seem like a worthwhile avenue for Big Science. "Big" because of the very large number of possibilities to test, in perhaps long-running experiments looking for low probability events. Worthwhile because creating life is miraculous.
Followups to the Miller-Urey experiment, searching for more complex structures.
Estimate more precisely one parameter of the Drake Equation.
There remains the much more difficult task of determining the parameters in which intelligent life will evolve.
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